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this result indicates that overexpression of serpina3n did not enhance the repair capacity of dystrophic myofibers, which is calcium dependent, but rather provided greater stability to the sarcolemma in the dystrophic myofibers from the onset, suggesting a mechanism whereby serpina3n overexpression leads to greater structural support of the membrane.
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the mouse serpina3n cdna was subcloned into a 3xfg vector from sigma to generate 3xfg-s3n that was then transfected into hek293 cells.
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